Peripheral nerve imaging
Use Cellvizio Lab to perform longitudinal studies on the peripheral nervous system.
In vivo imaging of degenerating axons and neuromuscular synapses
A: A small skin incision is made to expose the tibial nerve and the adjacent gastrocnemius muscle
B: intramuscular axons and motor nerve terminals in an innervated thy1.2-YFP16 mouse gastrocnemius muscle with an intact sciatic nerve.
C: Axons in the tibial nerve of thy1.2-YFP16 mice all degenerated by 3 days after section of the sciatic nerve, in contrast to the extensive axonal protection seen in WldS mouse nerves.
D: Axotomized tibial nerve axons are also preserved 3 days after sciatic nerve section in thy1.2-YFP16-WldS heterozygotes
E. Intact NMJs in the gastrocnemius muscle of a WldS homozygote were observable 3 days after sciatic nerve injury but,
F: No intact NMJs were ever seen in thy1.2-YFP/WldS heterozygotes at this time point.