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Pre-clinical research > Applications > Peripheral Nervous System

Peripheral Nervous System

 

          Results                         Perspectives                         Applications                       Bibliography

 

 

 

 

 

 

Peripheral Nerves

Imaging at work.

 

 

Cellvizio has been proven to be invaluable to peripheral nerve research, being able to image along nerves at a cellular level resolution and with minimal invasiveness to the animal.

 

 

This ensures high data reproducibility and fidelity in longitudinal studies.

 

  
 

 

    
Cutting-edge results

 

For the first time, imaging of neuromuscular junctions is made possible with Cellvizio Lab.

 

The video on the right shows motor endings of a YFP nerve stained with Rhodamine 6G

ProFlex™: MiniO/30

 Field of view : 400 x 280µm

Courtesy of Igor Charvet and Paolo Meda. CMU, Geneva, Switzerland

 

 

 

    

 

 

Imaging Nerve regeneration. Longitudinally.

 

Using our low invasive ProFlex, assessing nerve regeneration is possible, in vivo, over time.

This movie of the saphenous nerve was taken in vivo on the living mouse, after undertaking a small incision of the skin

 
 

 
     

 

Applications and Publications

 

Thanks to its high resolution, low invasiveness and ease-of-use, Cellvizio LAB was chosen by Wong et al.(MCN, 2009) to image slow axonal regeneration of neuromuscular junctions after nerve axotomy in transgenic mice.
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Within a time-lapse, in vivo imaging process, they were able to detect and monitor pathological signs of spontaneous synaptic degeneration in mice . Thus, Cellvizio LAB paves the way to the identification of the mechanisms involved into synaptic protection, and could therefore offer the capability to examin and monitor treatments from early-stages of neuromuscular disease.
 
 
  • Axonal and neuromuscular synaptic phenotypes in Wld(S), SOC1(G93A) and ostes mutant mice identified by fiber-optic confocal microendoscopy.

Wong F, Fan L, Wells S, Hartley R, Mackenzie FE, Oyebode O, Brown R, Thomson D, Coleman MP, Blanco G, Ribchester RR. Molecular & Cellular Neurosciences. (2009 Dec; 42(4): 296-307)

 

 

 

 

 

The Thy-1 H YFP strain (left) does not express YFP in every strand within the nerve bundle (compare with Thy-1 16 YFP strain on right).

This characteristic is also observed at the neuromuscular junction and can be exploited to repeatedly relocalize individual nerve termini.

Images courtesy of Dr. Richard Ribchester, University of Edinburgh

 



Cellvizio LAB enables time-lapsed acquisitions for minimally invasive longitudinal studies over days or even weeks.

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This unique feature has made is possible to obtain ground-breaking results on the quantification of degenerative and regenerative processes of a nerve after a lesion. Download our Application Note on this subject on the right-hand side of the page.Also, see the articles published in NeuroImage and EMBO Reports.

Left: Motor endplate or dendritic endings mapping is also at reach, as illustrated by the mosaic of images taken in vivo.







 





Perspectives


 

Cellvizio LAB can help you evaluate microvessel damages in diabetic neuropathies, with direct imaging of nerve microvascularization.

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Infiltration of immune cells, after adoptive transfer of GFP positive cells for instance, can also be imaged in vivo and quantified longitudinally.

Left : Sciatic nerve of a rat stained with acriflavine. Vessels irrigating the nerve are revealed by their negative contrast.

Courtesy of : Prof. Claudia Sommer, Neurologische Klinik der Universitaet, Wuerzburg, Germany

 

 

 

 

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