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Pre-clinical research > Applications > Deep Brain Imaging

Deep Brain Imaging

 

 

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Deep Brain Imaging

In vivo, in situ, in real-time.

 

 

 
     

 

Watching neurons at work at any area in the brain

with cellular resolution is a neuroscientist’s dream.

 

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That dream came true when Mauna Kea Technologies introduced 300 µm microprobes for deep brain imaging at the microscopic level. Cellvizio® Lab became the first off-the-shelf, easy to use solution for in vivo, in situ microscopic imaging of any location in the brain.

 

 

 

NeuroPak™ at a glance

 

 

Designed for Neuron imaging in freely behaving mouse

 

Now in vivo deep brain activity can be correlated

 to behavioral studies at the same time.

 

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Your Advantages for Brain Research.

 

 

 
  • Cellvizio sees deeper than any other microscope - including 2-photon

 

  • Cellvizio opens the possibility to reach any brain area in living animal models at the cellular level

 

  • Cellvizio offers qualitative and quantitative observations for numerous applications including :

 

  1. Gene expression
  2. Neuronal activity
  3. Stem cells /neuron precursors monitoring
  4. Vascularization

 

  • Cellvizio readily integrates into brain research workflow through stereotaxic access

 

  • Cellvizio combines high performances with very limited invasiveness and user friendly environment

 

 

 

 

Applications and Publications.

 

 

Ultra-thin ProFlex™ microprobes can routinely be used for in situ deep brain microscopic imaging on anesthetized animals.
 

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At only 300 microns in diameter, the insertion of the ProFlex™ microprobe induces minimal damage to the surrounding tissue and allow you to observe functioning cells in real-time, in their native environment.

 
The S-300 or S-650 ProFlex™ are readily compatible with any stereotaxic apparatus and can be lowered to any brain structure, cortical or sub-cortical.

 

 
 

Cellvizio Imaging for Neuroscience

 

Cellvizio allows fast, reliable, real-time acaquisition of deep brain cellular activity in situ.

 

► Find out ImageCell™, our advanced software
   
   
  • Gene expression

 

 

CaMK–eGFP transgenic mice

 

Medium-spiny neurons and surrounding neuropil in the striatum.

 

Penetration depth, 4 mm.

 

ProFlex™ used : S-300
   
   
  • Functional Imaging (Calcium imaging)

Changes in intracellular calcium of individual neurons in the visual cortex in response to visual stimuli.

 

 

 

 

Video courtesy of Clay Reid, Harvard Medical School.

   
   

  • Cellular Migration

 

Migrating neuron

 

Culture slice ex vivo, in response to ATP stimulation.

 

 

 

 

Video courtesy of Ingo Bechmann, Universitat Leipzig.
   
   
  • Assess Neuronal disorders by targeting the right area using ProFlex™ microprobes :

 

- Hippocampus : Alzheimer Disease

 

- Accumbens and VTA : Addictions

 

- Substantia Nigra : Parkinson
 
 

 

Cellvizio Lab for Neuroscience in Nature's EMBO Reports


The paper covers:


- Peripheral Nerve regrowth monitoring
- Deep brain imaging with a stereotaxic frame
- Deep brain with endoscopic access thru the nostril

- Calcium imaging of chemically-induced epileptiform seizures

   and electrically triggered synchronous neuronal activity.

 

 

 

 

 

 



You will find below other key papers and links highlighting the value of Cellvizio Lab for Neuroscience:

 

  • In vivo and in situ gene expression monitoring: J. Bergey et al. published in PNAS the possibility to check for gene expression after gene vectorization by nanoparticles to the left ventricle in a mouse brain.

 

  • P.M. Lledo et al. used Cellvizio® to monitor neuron precursor migration, as published in Chemical Senses

 

 

 

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