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Pre-clinical research > Applications > Pancreatic Cancer Imaging

Pancreatic Cancer Imaging

The pancreas has been the focus of many small animal studies with Cellvizio® LAB :

  • Microvasculature of normal vs. cancerous pancreatic tissue
  • Detection of pancreatic cancer in conjunction with wide field techniques
  • Usefulness of Cellvizio LAB for pancreatic cancer staging, through laparoscopic lymph node evaluation


These studies are translational by nature as shown in several papers :
 

  • Pancreatic cyst characterization through EUSFNA in pigs
  • Surgical assessment of cancerous tissue prior to treatment on rats


Here are examples of images and sequences obtained in the studies mentioned above :


Pancreas Histology

 


 Image of the pancreas obtained after cytoplasmic staining with Rhodamine 123

Field of view 400 x 280 µm
ProFlex S-1500

Courtesy of Igor Charvet and Paolo Meda, CMU, Geneva, Switzerland



Pancreatic acini

Field of view 240 x 240 µm
Proflex MiniO

Courtesy of Mauna Kea Technologies, Paris, France


Pancreas Vascularization



 Pancreatic vessels pool

Field of view 400 x 280 µm
ProFlex S-1500

Courtesy of Brice Mahé and Behazine Combadière, La Pitié Salpétrière, Paris, France


Early-stage Cancer Detection



  • Von Burstin et al. Highly sensitive detection of early-stage pancreatic cancer by multimodal near-infrared molecular imaging in living mice. Int. J. Cancer, 2008
 
 
Aim: Investigate a novel technique (combination of NIRF imaging and pCLE) for pancreatic cancer detection
Mouse model of pancreatic cancer
Development of fluorescent probes activated by specific proteases alteration (cathepsins and MMPs) specific for cancer
 
Results: Detection of tumors as small as 1-2mm
Application: Translation to clinic: improved detection of pancreatic cancer
 

 

Whole body NIRF Imaging and Cellvizio® LAB

c,d: Whole body NIRF imaging of a mouse model
of pancreatic tumor
Biomarker BxPC-3 (c), Biomarker MiaPaCa-2 (d)
e,g: Tumor imaged with Cellvizio® LAB
f: Healthy tissue imaged with Cellvizio® LAB
h: NIRF imaging of explanted pancreas and spleen

 


 


  • Lin et al. In vivo Quantitative Microvasculature Phenotype Imaging of Healthy and Malignant Tissues Using a Fiber-optic Confocal Laser Microprobe. Translational Oncology, 2008.
 

Aim: In vivo microscopic imaging of the microvasculature of normal vs. cancerous pancreatic tissue (ductal adenocarcinoma)
Longitudinal study: 7 mice examined over two weeks after tumor implantation (2 tumor lines)

Imaging with the Cellvizio® 660 system
Fluorescent staining: AngioSense 680, Visen Medical
Results: Differences in microvasculature between normal and cancerous tissue detected
 

 
Ex vivo haematoxylin and eosin stain

a: Normal acini and Islet of Langerhans structures

b,c: Cancerous tissue with lymphocytic infiltrates,
fibrotic stroma and loss of acinar structures

 

 


Tumor microvasculature

Cellvizio® 660 images in vivo and in situ the pancreatic microvasculature pattern
 

Staining: AngioSense 680 (Visen Medical)

 

ProFlex MiniO
 

 

 




 


  • Ignat et al. Feasibility and Reliability of Pancreatic Cancer Staging Using Fiberoptic Confocal Fluorescence Microscopy in Rats. Gastroenterology, 2009


Aim: Assess if Cellvizio can help in staging pancreatic cancer, by trying to identify cancer cells in the pancreas and in the surrounding lymph nodes
Rat model of pancreatic cancer (ductal adenocarcinoma)

Fluorescent staining: ProSense 660 (Visen Medical)
Results: Cellvizio LAB helped to identify very small metastases, initially missed by histopathology
Application: Improve outcomes of pancreatic cancer surgery, by a better detection of small metastases
 
 

 
Ductal Cells in the Pancreas

C: Image obtained with the Cellvizio®
D: H&E slide


 


Ductal Cells in the lymph nodes

E: Image obtained with the Cellvizio®
F: H&E slide		  





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