Pre-clinical research
GI Cancer research
Mice colonoscopies made easy
The exceptionally small size of ProFlex™ microprobes makes it an ideal tool for performing colonoscopies in mice, whether with a mice colonoscope, as shown on the video, or directly with the probe.
We show here an example where Cellvizio LAB led to the identification of normal and abnormal colonic crypts in the mucosa among two groups of mice: in the AOM-treated group, aberrant crypt foci (ACF) were clearly differentiated from normal, round shaped colonic crypts.
 |
 |
|
|
See also:
Pancreatic Cancer Imaging
Cellvizio LAB enabled the users to see them as brighter, bigger and elevated compared to the rest of the colon. These results were confirmed by standard histology after resection of the colon.
STxB-labeled tumors can be observed in force-fed anesthesized mice using confocal laser endoscopy.
- A: Experimental set-up
The ProFlex is introduced into the lumen of the small intestine. During experimentation, the probe is displaced along the intestinal mucosa (arrow).
- B: Confocal laser endoscopy on wild-type and transgenic mice.
Only isolated STxB-positive cells were observed by the Cellvizio in the intestine of wild-type mice (a).
In contrast, the tumor tissue in transgenic mice was strongly labeled (b)
- C: Immunostaining Results
Immunostaining with anti-STxB antibody on frozen sections prepared from the same animals as in (B) confirmed the results obtained by video-analysis.
Rare single cells were labeled in normal intestine (a), as observed in Fig. 2A and E. Glandular tumoral structures from transgenic animals were strongly stained (b), as observed in Fig. 2B and F.
See also:
Pancreatic Cancer Imaging
TOP
