In Vivo Microscopic Imaging of Transgenic Animals

Transgenic animals offer tremendous possibilities for in situ imaging, combining the best of genetic technologies and in vivo microscopic imaging with the Cellvizio.

Four different trangenic models are presented here, in the following order:

- the Thy1-YFP mouse: YFP expression in the PNS and subsets of the CNS

- the CamKinase-GFP mouse: GFP expression in some neuronal subsets

- the CNPase-GFP mouse: GFP oligodendrocytes and Schwan cells

- the Nagy mouse: ubiquitous GFP expression in various organs and muscles

Thy1-YFP mouse

Axon bundle in the sciatic nerve of a Thy1-YFP mouse

Field of view 400 x 280 µm
ProFlex S-1500, hand-held

Courtesy Igor Charvet, Medical Research Center (CMU), Geneva, Switzerland and Luc Stoppini, Biocell Interface, Geneva, Switzerland

Motor nerve terminals at the neuromuscular junction of Thy1-YFP mouse

Field of view 400 x 280 µm
ProFlex S-0650, hand-held

Courtesy Igor Charvet, Medical Research Center (CMU), Geneva, Switzerland and Luc Stoppini, Biocell Interface, Geneva, Switzerland

Neuromuscular junction of a Thy1-YFP mouse with skeletal muscle fibers marked by topical application of Syto13

Field of view 400 x 280 µm
ProFlex S-0650, hand-held

Courtesy Igor Charvet, Medical Research Center (CMU), Geneva, Switzerland and Luc Stoppini, Biocell Interface, Geneva, Switzerland

Dendritic receptors in a Thy1-YFP mouse, acquired on the reverse of a dorsal skin flap

Field of view 400 x 280 µm
ProFlex S-0650

Courtesy Igor Charvet and Paolo Meda, CMU, Geneva, Switzerland

Cell bodies and neurites in the dorsal root ganglion of a Thy1-YFP mouse

Field of view 400 x 280 µm
ProFlex S-0650


Courtesy Igor Charvet and Paolo Meda, CMU, Geneva, Switzerland

In vivo pyramidal neuron in the cortex of a Thy1 YFP mouse

Field of view 160*120 µm
ProFlex ProFlex HD-1800/80

Courtesy Prof. Johan Storm, Olve Moldestad, Gu Ning, Institute of Physiology, University of Oslo, Norway

Nerve fibers in the spinal cord of a Thy1-YFP mouse, after laminectomy

Field of view 400 x 280 µm
ProFlex S-0650

Courtesy Igor Charvet and Paolo Meda, CMU, Geneva, Switzerland

Certain of these Cellvizio applications and/or materials used with Cellvizio may require liensing under patents held by third parties. Users of Cellvizio imaging systems should consult with their counsel to determine whether licensing of any such third party patents may be required.

The Nagy and Thy1-YFP mouse models used in this Image Atlas are distributed by The Jackson Laboratory, Bar Harbor, Maine, USA: http://jaxmice.jax.org